Simplified DNA Quantification and Normalization
Summary
This article shows the use of FluentControl Smart Commands in this software to simplify and automate processing and calculations, while enhancing the ease of modification of the protocol to suit different workflows. The worktable was set up following the on-screen instructions of FluentControl software, and requested parameters were selected, some examples being shown in Figure 3: The full workflow was completed in 75 minutes including preparing an intermediate dilution plate as well as taking fluorescence readings in triplicate. As Table 1 shows, each replicate was analyzed for concentration in each of the five runs using linear regression techniques in compliance with the QuantiFlor dsDNA manual. In addition, when quantification values were cross-checked using 48 samples at high-concentration and low-concentration respectively in a checkerboard layout, the results showed no significant variation in any part of the plate ruling out any cross-contamination, as shown in Figure 5. Their systems and components help people working in clinical diagnostics, basic and translational research and drug discovery bring their science to life.
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