New Microplate-Based Method to Measure OCR and ECA Under Steady-State [O2]
Summary
“The fact that cells of internal tissues are not exposed to the ambient O2 tension has often been neglected for the sake of practical work and ease of use in the lab,” said Carl Peters, Application Specialist with BMG LABTECH.Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others for use as research reagents and qualified standards.Ann Arbor, MI, April 26, 2017 --( PR.com )-- Cayman Chemical, in collaboration with BMG LABTECH GmbH, has developed a novel assay kit to measure mitochondrial function under a dynamic range of oxygen concentrations.The ACU integrated into the CLARIOstar® reader facilitates measurement of cellular oxygenation at a controlled [O2], while phosphorescent extracellular O2 (MitoXpress® Xtra) and fluorescent extracellular pH (pH-Xtra™) probes provided in the kit enable multi-parametric detection of electron transport chain activity and glycolytic flux.By combining these advanced instrumentation and assay technologies, this kit eliminates the often confounding hyperoxic conditions under which most commercially available, cell-based in vitro assays are conducted.Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others for use as research reagents and qualified standards.