HoloBio: A holographic microscopy tool for quantitative biological analysis
Summary
HoloBio is a new open-source Python GUI for holographic microscopy analysis in biology and biomedical imaging. It supports both real-time and offline workflows for DHM and DLHM data, including reconstruction, phase compensation, refocusing, and filtering. The software also adds biology-focused tools for cell counting, thickness estimation, morphology analysis, speckle reduction, and particle tracking. The article demonstrates use cases with red blood cells, onion epidermis tissue, paramecia, and live tracking videos. The release is aimed at researchers who need accessible, reproducible analysis software without coding expertise.
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