New Ampli-Fi ultra-low-input protocol will support HiFi sequencing from as little as 1 ng of DNA
Summary
The latest SPRQ chemistry update on the Revio system enables long-read sequencing from as little as 500 ng of DNA, marking a pivotal moment for researchers studying complex genomes. The new Ampli-Fi workflow represents the latest evolution in PacBio’s low-input solutions for scientists studying the world around us, from the tiniest species on the tree of life to preserved specimens from ancient eras of history. In another study, (Männer et al., 2024) use the KOD Xtreme Hot Start DNA polymerase protocol to generate a chromosome-level genome assembly and annotation of the marine sacoglossan sea slug, Elysia timida.2 This assembly resulted in nearly 20,000 protein-coding genes, which are more contiguous and complete compared to other publicly available annotations of Sacoglossa. • Bein B, Chrysostomakis I, Arantes LS, et al. Long-read sequencing and genome assembly of natural history collection samples and challenging specimens. bioRxiv 2024.03.04.583385; doi: https://doi.org/10.1101/2024.03.04.583385 • Männer L, Schell T, Spies J, et al. Chromosome-level genome assembly of the sacoglossan sea slug Elysia timida (Risso, 1818).