Unlocking cardiac motion: assessing software and machine learning for single-cell and cardioid kinematic insights - Scientific Reports
Summary
According to its nature, MM can report the average kinematics of the sample, highly dependable on the quality of image intensity and resolution, with the ability to perform a single pixel movement evaluation, as stated in the work of Sala et al.36. As in CW, an updated feature of the ViKiE software allows the user to indicate the starting and ending point for each beat to calculate the duration, as well as the time to reach the peak of both contraction and relaxation phases on the speed profile. Once the raw data were acquired during the experiments described in detail below, the post-processing of the kinematics was performed with the listed open-source programs and the absolute values of speed profiles were compared with the parameters displayed graphically in Fig. Moreover, it has to be noted that the profiles reported throughout the work will be indicated as absolute values to avoid misinterpretation and ensure consistency in data representation of the outputs from the three computer vision software. On the 26th day of culture, the cells were purified from all non-cardiomyocytes by MACS PSC-Derived Cardiomyocyte Isolation Kit (Miltenyi Biotec, #130-110-188) as the negative fraction to depletion antibodies.