MoBiTec GmbH, Germany, Introduces New Multiplex Secretion Gene Expression System for Bacillus subtilis
Summary
With its ability to secrete protein directly into culture media, and designation by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe (GRAS), the majority of industrially-produced enzymes are expressed in Bacillus species such as B. subtilis.This enables customers to perform a downstream assay to identify and select clones which secrete the highest amount of functional protein into the culture media, and quickly identify the signal peptide that results in efficient expression of the desired secreted protein.subtilis shuttle vector with B. subtilis-derived subtilisin (aprE) promoter, secretory signal peptide (aprE SP), Multiple Cloning Site, and 3 (C-terminal) His-tag sequence · Supplied with SP DNA Mixture, a library of DNA sequences encoding 173 unique secretory signal peptides that can be inserted upstream of your target gene · Fully compatible with Fuse-In Cloning Kit (PBS104) to allow rapid and easy construct generation · Includes B. subtilis strain RIK1285 · Premium Competent E. coli HST08 cells for cloning available as well (PBS105) For more information including manuals visit our website: http://www.mobitec.de/int/products/bio/04_vector_sys/index.php?bac_sub-multi.html Goettingen, Germany, December 13, 2012 --( PR.com )-- For the economical production of recombinant proteins, the existence of stable expression systems is a necessity.This enables customers to perform a downstream assay to identify and select clones which secrete the highest amount of functional protein into the culture media, and quickly identify the signal peptide that results in efficient expression of the desired secreted protein.Features· Includes pBE-S DNA, an E. coli/B.subtilis shuttle vector with B. subtilis-derived subtilisin (aprE) promoter, secretory signal peptide (aprE SP), Multiple Cloning Site, and 3 (C-terminal) His-tag sequence· Supplied with SP DNA Mixture, a library of DNA sequences encoding 173 unique secretory signal peptides that can be inserted upstream of your target gene· Fully compatible with Fuse-In Cloning Kit (PBS104) to allow rapid and easy construct generation· Includes B. subtilis strain RIK1285· Premium Competent E. coli HST08 cells for cloning available as well (PBS105)For more information including manuals visit our website:http://www.mobitec.de/int/products/bio/04_vector_sys/index.php?bac_sub-multi.html