Tangential-Flow Filtration Process Control
Summary
The system’s features enable its implementation in multiple unit operations and biopharmaceutical processing applications, including ultrafiltration–diafiltration (UF–DF) of proteins, viruses, and compounds such as oligonucleotides and antibody–drug conjugates (ADCs). The control system includes PendoTECH single-use pressure sensors, which are available with 1/8-inch to one-inch hose-barb connections and in a luer fitting and sanitary flange (triclamp, TC) format to handle all process scales while minimally influencing holdup volume. The system has no process-scale limitations, thereby enabling users to scale up or down easily with minimal outlay and space requirements as a process advances from a benchtop to pilot-scale setup. The Mettler Toledo M300 transmitter can read high-sensitivity probes and be integrated with one of the generic configurable inputs to the PendoTECH TFF control system (Figure 2). The TFF operations for ADCs typically are done in a biological safety cabinet (hood) to reduce human exposure to toxic components linked to the antibody.